L-asparaginase is known to be the most hopeful and upcoming anti-tumour enzyme helpful in reduction of the level of amino acid L-asparagine resulting in cancer cell starvation and leading to the cell death. Although, there are many sources of asparaginase enzyme yet bacterial source i.e. E.coli and Erwinia species is mostly used as a therapeutic agent against leukaemia. In presented study screening of various bacteria is carried out by rapid plate assay for asparaginase. Enzyme producing then intracellular and extracellular Asparaginase were extracted from its potent producer i.e. E.coli that is isolated from sewage water. Obtained results showed that an intracellular asparaginase enzyme is found to be more active against asparagines as compare to the extracellular enzyme.
This is a pilot study with a preliminary demonstration of safety and efficacy of FSCs administration in children with CP. This research has shown that FSCs therapy, irrespective of CP severity, favourably influences the course of children’s development and their immunological markers. Such future reflective research studies are essential as well as larger randomized, placebo-controlled trials would help to further characterize potential of FSCs associated improvements in children with СР.
One of the basic fundamental rights of patients is the autarchy to decline medical treatment. An important feature of medical ethics is the right to decline and honour the wishes of the patients. Even though advanced decisions are not immutable and can or may be negotiated by family members or any loved ones as decision makers. Author describes a case that highlights the significance of advanced care planning, complexity of decision making, and area for potential improvements in clinical field.
Mrs. Smith, whose named has been changed for privacy purposes, was an elderly woman, well into her 80s, with diabetes, hypertension, hyperlipidemia, peripheral vascular disease, heart failure, and chronic obstructive pulmonary disease. She was my clinic patient and has been followed for many years in our resident practice. Her daughter always brought her to her appointments, and waited politely in the waiting room while her mom saw the doctor.
Medicinal plants show great interest today because of its multiple physiological effects. They contain a diversity of chemical compounds, phytochemicals, with demonstrated important biological activities. These compounds are produced by the secondary metabolism of plants, which have evolved from different biotic or abiotic factors, and used by the plant for defense or survival. However, multiple studies on these compounds have proven beneficial to human health.
Phytochemicals have multiple properties such as antioxidant and anti-inflammatory, which can be used as a preventive means to protect against the development of health problems. They may interact with molecules that can inhibit the genesis of this type of pathologies or conversely, interact with biomarkers that enhance the protective capabilities of the organism.
Recombinant antibody technology progressed rapidly during last decade, mainly because of their human therapeutic use. The vertebrate immune system generates billions antibody molecules. Of them, camelids have been found to possess structurally different kinds of antibodies. These camelid antibodies are composed of two heavy chains similar to conventional antibodies.
Contrary to the structure found in conventional antibodies these heavy-chain antibodies lack the CH1 domain. Nanobodies are antibody-derived engineered therapeutic proteins. Nanobodies possess structural and functional properties of naturally occurring heavy chain antibodies. The nanotechnology was originally developed after the discovery that camelidae (camels & llamas) possess fully functional antibodies without light chains. These antibodies contain a single variable domain (VHH) and two constant domains (CH2 and CH3).
Chen et al.demonstrated that curli (a natural bacterial amyloid fibril) expressed by many Enterobacteriaceae, including E. coli, was capable of enhancing alpha-synuclein (AS) aggregation in both aged rats and the nematode C. elegans overexpressing alpha-synuclein. They suggested that amyloid proteins in the microbiota might be involved in the triggering of neurodegenerative diseases.
In this letter, we wish to draw attention to the wide potential for reciprocal fertilization of experimental and observational research in organ-limited amyloid disorders. By way of reference, we take our recent review on the epidemiology of different conformational neurodegenerative disorders (CNDD) undertaken from a biological perspective, which incorporated descriptive and analytical elements of both the general and clinical epidemiology of CNDD.
Intestinal evacuation is a complex sequence of events resulting from the integration of the autonomic and somatic nervous systems and culminating in the elimination of fecal matter contained in the descending colon and rectum. In turn, intestinal habitus is a personal characteristic varying considerably among individuals due to factors such as quality and quantity of ingested food and drink, convenience and a satisfied feeling of well-being, with no discomfort and without the sensation that evacuation was not sufficient.
Functional gastrointestinal changes are relatively common disorders in various age ranges and may progress to chronic problems with repercussions on quality of life that lead to anxiety, depression, sexual insatisfaction, incontinence, hemorrhoids, anal fissures, and somatization of other problems. Important among these functional changes is functional constipation, which may reach a rate of up to 40% among children and adolescents and which is related to factors such as eating habits, socioeconomic level, and regional, racial and cultural differences.