Rhizobium, a soil inhabitant is a gram negative, motile, rod shaped bacterium that establishes a symbiotic association with the host legume by forming nodules on the surface of root. For the establishment and functioning of Rhizobium-legume symbiosis, coordinated expression of the several symbiotic genes of both partners is required.
A transposon is a DNA fragment which can transpose from one site to another in the genome. One of the applications of the transposon is to mutate a gene (mutagenesis) and determine the physical location of genes of interest. Mung bean (Vigna radiata) is an important legume and a wellknown economic crop in tropical and subtropical countries. It is often included in crop rotation to replenish nitrogen and improve soil fertility. Mung bean is used in several food products. It is rich in vitamin and minerals which are necessary for human body.