For any bacterium to propagate for any purpose it is necessary to provide the appropriate biochemical and biophysical environment. The food base that supports the growth of an organism called culture medium; the biochemical (nutritional) environment made available in this culture medium. The food base depending upon the special needs of particular bacteria (as well as particular investigators), so that a large variety and types of culture media have been developed with different purposes and uses. These include sources of organic carbon, nitrogen, phosphorus, sulfur and metal ions including iron. Culture media employed in the isolation and maintenance of pure cultures of bacteria and used for identification of bacteria according to their biochemical and physiological properties.
Grapes (Vitis vinifera ) provide many nutrients like carbohydrates (glucose), vitamins, minerals, fibers, phytochemicals and antioxidants. The functional quality of grape fruit characterized by its metabolic compositions. It contains a number of secondary metabolites like flavonols, anthocyanins, proanthocyanidins, stilbene derivatives . The minerals iron, potassium, zinc, manganese, and calcium were present in higher concentrations.