The Quantiferon®-TB Gold In-Tube Assay Detects Interferon

One of the remarkable advances in the diagnosis of tuberculosis (TB) in the last few decades has been the development and wide usage of interferon (IFN)-γ release assays (IGRAs). These assays quantify the in vitro release of IFN-γ from T-cells stimulated with Mycobacterium tuberculosis (M. tuberculosis) antigens. The innovation, together with their prevailing employment, facilitates the early identification of at-risk individuals allowing for early intervention treatment for latent TB infection, thereby preventing the spread of TB in health care settings and communities where the TB patients reside.

The Quantiferon®-TB Gold
The Quantiferon®-TB Gold

Two major IGRAs are widely utilized;one is QuantiFERON®-TB Gold In-Tube (QFT-GIT) (Cellestis, Victoria, Australia) and the other is T-SPOT.TB assay (Oxford Immunotec, Abingdon, United Kingdom). IGRAs, although not recommended for diagnosis of active TB in low- income countries, have been used to rule out latent TB infection especially for individuals who have received BCG or screening of health care workers.QFT-GIT simultaneously employs three M. tuberculosis antigens, ESAT-6, CFP-10, and TB7.7, to which cell- mediated immune responses are measured in a single tube.QFT- GIT replaces QuantiFERON®TB-Gold (QFT-G), which was the former generation of QuantiFERON applying two M. tuberculosis antigens with a separate reaction.

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